期刊
JOURNAL OF CELL SCIENCE
卷 128, 期 23, 页码 4293-4305出版社
COMPANY OF BIOLOGISTS LTD
DOI: 10.1242/jcs.164509
关键词
Ureteric bud; Metanephric mesenchyme; GTPase; Signaling; Epithelial cell
类别
资金
- VA Merit Reviews [1I01BX002025, 1I01BX002196]
- National Institutes of Health [R01-DK083187, R01-DK075594, R01-DK383069221, R01-DK095761, RO1-DK080004, RO1-DK095057, R01-DK099478, 5P30DK-079328]
- March of Dimes grants
The Rho GTPase Cdc42 regulates key signaling pathways required for multiple cell functions, including maintenance of shape, polarity, proliferation, migration, differentiation and morphogenesis. Although previous studies have shown that Cdc42 is required for proper epithelial development and maintenance, its exact molecular function in kidney development is not well understood. In this study, we define the specific role of Cdc42 during murine kidney epithelial tubulogenesis by deleting it selectively at the initiation of ureteric bud or metanephric mesenchyme development. Deletion in either lineage results in abnormal tubulogenesis, with profound defects in polarity, lumen formation and the actin cytoskeleton. Ultimately, these defects lead to renal failure. Additionally, in vitro analysis of Cdc42-null collecting duct cells shows that Cdc42 controls these processes by regulating the polarity Par complex (Par3-Par6-aPKC-Cdc42) and the cytoskeletal proteins N-Wasp and ezrin. Thus, we conclude that the principal role of Cdc42 in ureteric bud and metanephric mesenchyme development is to regulate epithelial cell polarity and the actin cytoskeleton.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据