期刊
CRYOBIOLOGY
卷 40, 期 1, 页码 84-89出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/cryo.1999.2225
关键词
liver; transplantation; antifreeze proteins; freezing; cryopreservation; glycerol
We transplanted rat livers cryopreserved at high subzero temperatures with a protocol that mimics freezing in freeze-tolerant animals. The results of nine transplants show that: (a) every single transplanted liver produced bile, which suggests that the cryopreserved livers retained some physiological function; (b) eight of the animals survived between 2 and 4 h with loss of microvascular integrity which suggests that transplantation failure is related to the circulation and tests of bile production are not indicative of transplantation success; and (c) one animal survived for 5 days with an intact circulation which might be due to an unidentified technical variation or could indicate that when the circulation recovers animals with transplanted livers survive, (C) 2000 Academic Press.
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