期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 66, 期 2, 页码 684-687出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.66.2.684-687.2000
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The conversions of vanillic acid and O-benzylvanillic acid to vanillin were examined by using whole cells and enzyme preparations of Nocardia sp. strain NRRL 5646. With growing cultures, vanillic acid was decarboxylated (69% yield) to guaiacol and reduced (11% yield) to vanillyl alcohol. In resting Nocardia cells in buffer, 4-O-benzylvanillic acid was converted to the corresponding alcohol product without decarboxylation. Purified Nocardia carboxylic acid reductase, an ATP and NADPH-dependent enzyme, quantitatively reduced vanillic acid to vanillin. Structures of metabolites were established by H-1 nuclear magnetic resonance and mass spectral analyses.
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