期刊
ANALYTICA CHIMICA ACTA
卷 860, 期 -, 页码 83-88出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aca.2014.12.031
关键词
Ochratoxin A; Hybridization chain reaction; Aptamer; Colorimetric method
资金
- NSFC [21375050, 21305032, 21305052]
- China Postdoctoral Science Foundation [2014M551522]
- Research Foundation of Jiangsu University [13JDG069]
- Qing Lan Project
The combination of high selectivity of aptamer with the peroxidase-mimicking property of DNAzyme has presented considerable opportunities for designing colorimetric aptasensor for detection of ochratoxin A (OTA). The activities of both aptamer (as biorecognition element) and DNAzyme (as signal amplification element) are blocked via base pairing in the hairpin structure. Hybridization chain reaction (HCR) between two hairpin DNAswas employed to further improve the sensitivity of this method. The presence of OTA triggers the opening of the hairpin structure and the beginning of HCR, which results in the release of many DNAzyme, and generates enhanced colorimetric signals, which is correlated to the amounts of OTA with linear range between 0.01 to 0.32 nM, and the limit of detection is 0.01 nM under optimal conditions. OTA in yellow rice wine and wheat flour samples was also detected using this method. We demonstrate that a new colorimetric method for the detection of OTA has been established, which is simple, easy to conduct, label-free, sensitive, high throughput, and cost-saving. (C) 2014 Elsevier B.V. All rights reserved.
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