期刊
MOLECULAR BREEDING
卷 6, 期 1, 页码 67-72出版社
SPRINGER
DOI: 10.1023/A:1009658128964
关键词
Agrobacterium tumefaciens; genetic transformation; herbicide resistance; crucifers; Brassica rapa; in planta transformation; in vivo selection
Transgenic pakchoi (Brassica rapa L. ssp. chinensis) plants were obtained in the progeny of plants infiltrated by an Agrobacterium tumefaciens strain carrying a gene for resistance to the herbicide phosphinotricin (Basta). Genetic analysis demonstrates the transmission of the herbicide resistant trait to the progeny. Molecular analyses show that the transgene was inserted in the plant genome and expressed. This work demonstrates that the infiltration transformation method originally devised for Arabidopsis thaliana can be adapted for other crucifer species and opens up the possibility of genetic engineering of pakchoi, an important vegetable plant.
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