期刊
JOURNAL OF STRUCTURAL BIOLOGY
卷 129, 期 1, 页码 80-95出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1006/jsbi.1999.4200
关键词
monosialoganglioside-G(M1); Marburg's syndrome; multiple sclerosis; myelin basic protein; scanning transmission electron microscopy; single particle image analysis; transmission electron microscopy; three-dimensional reconstruction
Myelin basic protein (MBP) is considered to be essential for the maintenance of stability of the myelin sheath, Reduction in cationicity of MBP, especially due to conversion of positively charged arginine residues to uncharged citrulline (Cit), has been found to be associated with multiple sclerosis (MS). Here, the interactions of an anionic phosphatidylserine/monosialoganglioside-G(M1) (4:1, w:w) lipid monolayer with 18.5-kDa MBP preparations from age-matched adult humans without MS (no Cit residues), with chronic MS (6 Cit), and with acute Marburg-type MS (18 Cit) were studied by transmission and ultralow dose scanning transmission electron microscopy under cryogenic conditions. Immunogold labeling and single particle electron crystallography were used to define the nature of the complexes visualized. These electron microscopical analyses showed that the three different MBP charge isomers all formed uniformly sized and regularly shaped protein-lipid complexes with G(M1), probably as hexamers, but exhibited differential association with and organization of the lipid. The least cationic Marburg MBP-Cit(18) formed the most open protein-lipid complex. The data show a disturbance in lipid-MBP interactions at the ultrastructural level that is related to degree of citrullination. and which may be involved in myelin degeneration in multiple sclerosis. (C) 2000 Academic Press.
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