期刊
JOURNAL OF BIOMOLECULAR NMR
卷 16, 期 2, 页码 139-146出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1008394910612
关键词
flow injection; protein; screening
In this technical note we describe the setup and application of automated sample preparation and usage of flow-through NMR equipment for the characterization of ligand binding on proteins. In addition, we focus on the perspectives of automated analysis of 2D HSQC spectra to identify changes in patterns indicative for ligand binding or changes of sample conditions. In this context we discuss a combination of statistical and non-statistical data analysis.
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