期刊
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
卷 5, 期 1, 页码 30-35出版社
SPRINGER VERLAG
DOI: 10.1007/s007750050004
关键词
electron paramagnetic resonance; magnetic circular dichroism; Raman spectroscopy; azurin; copper
The simultaneous excitation of a paramagnetic sample with optical (laser) and microwave radiation can cause an amplitude or phase modulation of the transmitted light at the microwave frequency. The detection of this modulation indicates the presence of coupled optical and electron paramagnetic resonance (EPR) transitions in the sample. Here we report the first application of this technique to a biomolecule: the blue copper centre of Pseudomonas aeruginosa azurin, Using optical excitation at 686 nm, in the thiol to copper(II) charge transfer band, we measure a coherent Raman-detected EPR spectrum of a frozen aqueous solution. Its lineshape is characteristic of the magnetic circular dichroism along each principal g-value axis. This information allows electronic and structural models of transition metal ion centres in proteins to be tested.
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