期刊
JOURNAL OF CELL BIOLOGY
卷 208, 期 2, 页码 223-237出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201409036
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资金
- National Institutes of Health [GM110413]
- University of Alabama Hepato-Renal Fibrocystic Disease Core Center (National Institutes of Health) [5P30DK074038-09, 0004392208-003]
- University of Georgia
T he assembly of the axoneme, the structural scaffold of cilia and flagella, requires translocation of a vast quantity of tubulin into the growing cilium, but the mechanisms that regulate the targeting, quantity, and timing of tubulin transport are largely unknown. In Chlamydomonas, GFP-tagged alpha-tubulin enters cilia as an intraflagellar transport (IFT) cargo and by diffusion. IFT-based transport of GFP-tubulin is elevated in growing cilia and IFT trains carry more tubulin. Cells possessing both nongrowing and growing cilia selectively target GFP-tubulin into the latter. The preferential delivery of tubulin boosts the concentration of soluble tubulin in the matrix of growing versus steady-state cilia. Cilia length mutants show abnormal kinetics of tubulin transport. We propose that cells regulate the extent of occupancy of IFT trains by tubulin cargoes. During ciliary growth, IFT concentrates soluble tubulin in cilia and thereby promotes elongation of the axonemal microtubules.
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