期刊
JOURNAL OF IMMUNOLOGY
卷 164, 期 3, 页码 1260-1268出版社
AMER ASSOC IMMUNOLOGISTS
DOI: 10.4049/jimmunol.164.3.1260
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CD5 positively costimulates TCR-stimulated mature T cells, whereas this molecule has been suggested to negatively regulate the activation of TCR-triggered thymocytes. We investigated the effect of CD5 costimulation on the differentiation of CD4(+)CD8(+) thymocytes. Coligation of thymocytes with anti-CD3 and anti-CDS induced enhanced tyrosine phosphorylation of LAT (linker for activation of T cells) and phospholipase C-gamma (PLC-gamma) compared with ligation with anti-CD3 alone. Despite increased phosphorylation of PLC-gamma, this treatment down-regulated Ca2+ influx. In contrast, the phosphorylation of LAT and enhanced association with Grb2 led to activation of extracellular signal-regulated kinase (ERK) mitogen-activated protein kinase. When CD3 and CD5 on CD4(+)CD8(+) thymocytes in culture were coligated, they lost CD8, down-regulated CD4 expression, and induced CD69 expression, yielding a CD4(+(dull))CD8(-)CD69(+) population. An ERK inhibitor, PD98059, inhibited the generation of this population. The reduction of generation of CD4(+)CD8(-) cells resulted from decreased survival of these differentiating thymocytes. Consistent with this, PD98059 inhibited the anti-CD3/CD5-mediated Bcl-2 induction. These results indicate that CD5 down-regulates a branch of TCR signaling, whereas this molecule functions to support the differentiation of CD4(+)CD8(+) thymocytes by up-regulating another branch of TCR signaling that leads to ERK activation.
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