期刊
JOURNAL OF INVESTIGATIVE DERMATOLOGY
卷 114, 期 2, 页码 334-342出版社
BLACKWELL SCIENCE INC
DOI: 10.1046/j.1523-1747.2000.00874.x
关键词
cyclic AMP; famotidine; pigmentation; tyrosinase
类别
Hyperpigmentation frequently accompanies chronic or acute inflammation. A number of inflammatory mediators have been shown to stimulate melanin synthesis in human melanocytes. Although histamine is ubiquitous as an inflammatory factor, its involvement in pigmentation remains obscure. In this work, we examined the effects of histamine on cultured human melanocytes, Treatment of human melanocytes with 0.1-10 mu M histamine evoked morphologic changes and increases in tyrosinase activity. The concomitant increases in melanin content of the histamine-treated melanocytes indicated an elevation of melanin synthesis by tyrosinase activation. These stimulatory effects of histamine mere completely inhibited by an H-2 antagonist, famotidine, whereas H-1 and H-3 antagonists had no inhibitory effect whatsoever, In addition, an H-2 agonist, dimaprit, induced the same degree of melanogenesis as histamine at concentrations of 0.1-10 mu M. We observed an increase in the intracellular cAMP contents of human melanocytes induced by histamine via the H-2 receptors, We know that this cAMP accumulation and subsequent protein kinase A activation plays a critical role in histamine-induced melanogenesis, because a specific protein kinase A inhibitor, H-89, completely suppressed these stimulatory effects of histamine, and because dibutylic cAMP, a specific protein kinase A activator, stimulated human melanocytes as potently as histamine. Taken together, we show here that histamine induces melanogenesis of human cultured melanocytes by protein kinase A activation via H-2 receptors.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据