期刊
BIOMEDICAL MICRODEVICES
卷 16, 期 4, 页码 537-548出版社
SPRINGER
DOI: 10.1007/s10544-014-9856-2
关键词
Circulating tumor cells (CTCs); Cancer cell isolation; Metastasis; Negative enrichment; White blood cell (WBC) depletion
资金
- Science and Engineering Research Council of A*STAR (Agency for Science, Technology and Research), Singapore [1031490005]
Negative enrichment is the preferred approach for tumor cell isolation as it does not rely on biomarker expression. However, size-based negative enrichment methods suffer from well-known recovery/purity trade-off. Non-size based methods have a number of processing steps that lead to compounded cell loss due to extensive sample processing and handling which result in a low recovery efficiency. We present a method that performs negative enrichment in two steps from 2 ml of whole blood in a total assay processing time of 60 min. This negative enrichment method employs upstream immunomagnetic depletion to deplete CD45-positive WBCs followed by a microfabricated filter membrane to perform chemical-free RBC depletion and target cells isolation. Experiments of spiking two cell lines, MCF-7 and NCI-H1975, in the whole blood show an average of > 90 % cell recovery over a range of spiked cell numbers. We also successfully recovered circulating tumor cells from 15 cancer patient samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据