期刊
JOURNAL OF CELL BIOLOGY
卷 209, 期 5, 页码 633-644出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201409074
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资金
- Center for Nanoscience, Nanosystems Initiative Munich
- Deutsche Forschungsgemeinschaft [SPP 1623 CA 198/8-1, HE 721/13-1]
- AFFINOMICS (Protein Binders for Characterization of Human Proteome Function: Generation, Validation, Application), Seventh Framework program [241481]
Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes.
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