期刊
BIOMEDICAL MICRODEVICES
卷 15, 期 4, 页码 699-709出版社
SPRINGER
DOI: 10.1007/s10544-013-9757-9
关键词
Circulating tumor cells (CTCs); Cancer cell isolation; Metastasis; Negative enrichment; White blood cell (WBC) depletion
资金
- Science and Engineering Research Council of A*STAR (Agency for Science, Technology and Research), Singapore [1031490005]
Our current understanding of clinical significance or the lack thereof of circulating tumor cells (CTCs) is biased by the technology used to isolate these rare cells. Despite the presence of a vast number of academic and commercial technologies, the lack of a standardized and optimized platform has been widely noted. We present a negative enrichment approach, integrating WBC depletion and chemical-free RBC depletion in the same setup without the need for centrifugation, washing or multiple sample handling steps. This approach achieves an average of >90 % recovery of spiked tumor cells and >99 % total WBC depletion in whole blood across multiple cell lines, in a simple and easy-to-use assay. The results presented herein and ongoing improvements aim to fulfill the need for a highly reliable, unbiased, standardized, and optimized CTC isolation platform, using component technologies that are validated for cell isolation.
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