期刊
BIOMEDICAL MICRODEVICES
卷 14, 期 6, 页码 1019-1025出版社
SPRINGER
DOI: 10.1007/s10544-012-9704-1
关键词
Poly(ethylene glycol); Salmonella typhimurium; Patterning; Poly-L-lysine; Microrobot
资金
- Future Pioneer R&D program through the National Research Foundation of Korea
- Ministry of Education, Science, and Technology [2012-0001035]
- National Research Foundation of Korea [2009-0082954] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
For the development of bacteria-based biomedical microrobot, we propose the fabrication method of biocompatible poly(ethylene glycol) (PEG) microbeads using a cross-junction microfluidic channel. PEG droplets were polymerized by ultraviolet (UV) irradiation to form PEG microbeads of 8.18 +/- 3.4 mu m diameter in a microfluidic channel. Generally, the bacteria did not attach to the surface of the PEG microbeads because of their hydrophilicity. We modified the selective surface of the PEG microbeads using poly-L-lysine (PLL), promoting attenuated Salmonella typhimurium adhesion using the submerging property of PEG microbeads on agarose gel: the bacteria could thus be attached to the PLL-coated surface region of the PEG microbeads. The selectively PLL-coated PEG microbeads group showed enhanced motility compared with the PLL-uncoated and completely PLL-coated PEG microbeads groups. The selectively PLL-coated PEG microbeads group showed 12.33 and 7.40 times higher average velocities than the PLL-uncoated and completely PLL-coated PEG microbeads groups, respectively. This study verified the successful fabrication of bacteria-based microrobots using PEG microbeads, and the enhanced motility of the microrobots by selective bacteria patterning using agarose gel and PLL.
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