期刊
JOURNAL OF CELL BIOLOGY
卷 211, 期 1, 页码 53-61出版社
ROCKEFELLER UNIV PRESS
DOI: 10.1083/jcb.201504042
关键词
-
类别
资金
- British Heart Foundation
- EU Marie Curie Fellowship
- UK MRC
- MRC [MC_U105178778, G0800784, G1000847] Funding Source: UKRI
- British Heart Foundation [RG/13/4/30107, PG/13/91/30579, PG/14/25/30726] Funding Source: researchfish
- Medical Research Council [MC_U105178778, G0800784, G1000847] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0514-10086] Funding Source: researchfish
Caveolae are strikingly abundant in endothelial cells, yet the physiological functions of caveolae in endothelium and other tissues remain incompletely understood. Previous studies suggest a mechanoprotective role, but whether this is relevant under the mechanical forces experienced by endothelial cells in vivo is unclear. In this study we have sought to determine whether endothelial caveolae disassemble under increased hemodynamic forces, and whether caveolae help prevent acute rupture of the plasma membrane under these conditions. Experiments in cultured cells established biochemical assays for disassembly of caveolar protein complexes, and assays for acute loss of plasma membrane integrity. In vivo, we demonstrate that caveolae in endothelial cells of the lung and cardiac muscle disassemble in response to acute increases in cardiac output. Electron microscopy and two-photon imaging reveal that the plasma membrane of microvascular endothelial cells in caveolin 1(-/-) mice is much more susceptible to acute rupture when cardiac output is increased. These data imply that mechanoprotection through disassembly of caveolae is important for endothelial function in vivo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据