期刊
BIOMEDICAL MICRODEVICES
卷 12, 期 6, 页码 987-1000出版社
SPRINGER
DOI: 10.1007/s10544-010-9453-y
关键词
Shear activation; Microfluidics; Platelet-surface interactions; Protein characterisation; Image analysis
资金
- Science Foundation Ireland [05/CE3/B754]
- Science Foundation Ireland (SFI) [05/CE3/B754] Funding Source: Science Foundation Ireland (SFI)
We report a novel device to analyze cell-surface interactions under controlled fluid-shear conditions on well-characterised protein surfaces. Its performance is demonstrated by studying platelets interacting with immobilised von Willebrand Factor at arterial vascular shear rates using just 200 mu L of whole human blood per assay. The device's parallel-plate flow chamber, with 0.1 mm(2) cross sectional area and height-to-width ratio of 1:40, provides uniform, well-defined shear rates along the chip surface with negligible vertical wall effects on the fluid flow profile while minimizing sample volumetric flow. A coating process was demonstrated by ellipsometry, atomic force microscopy, and fluorescent immunostaining to provide reproducible, homogeneous, uniform protein layers over the 0.7 cm(2) cell-surface interaction area. Customized image processing quantifies dynamic cellular surface coverage vs. time throughout the whole-blood-flow assay for a given drug treatment or disease state. This device can track the dose response of anti-platelet drugs, is suitable for point-of-care diagnostics, and is designed for adaptation to mass manufacture.
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