Peroxisome proliferator-activated receptors are expressed in mouse bone marrow-derived mast cells

We examined the expression of peroxisome proliferator-activated receptors (PPARs) and the role of PPARs in cytokine production in mouse hone marrow-derived mast cells (mBMMCs). mBMMCs expressed PPAR beta strongly and gamma slightly, but not a. Activation of mBMMCs with antigen or calcium ionophore resulted in the increased expression of PPAR gamma mRNA specifically. 15-Deoxy-Delta(12,14)-prostaglandin J(2) (15d-PCJ(2)) and troglitazone, all PPAR gamma ligands, attenuated the antigen-induced cytokine production by mBMMCs. Carbaprostacyclin, a PPAR beta ligand, also inhibited cytokine production, whereas PPAR alpha ligands did not. These results suggest that PPAR beta and gamma might be included in the negative regulation of mast cell activation. (C) 2000 Federation of European Biochemical Societies.