期刊
JOURNAL OF BIOLOGICAL CHEMISTRY
卷 275, 期 6, 页码 4099-4103出版社
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
DOI: 10.1074/jbc.275.6.4099
关键词
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资金
- NIGMS NIH HHS [GM34558] Funding Source: Medline
In order to define regions of ParE, one of the two subunits of topoisomerase IV, that are involved in catalysis during topoisomerization, we developed a selection procedure to isolate dominant-negative parE alleles. Both wild-type parC and mutagenized parE were expressed from a tightly-regulated lac promoter on a moderate-copy plasmid, Mutated parE alleles were rescued from those plasmids that caused IPTG-dependent cell death, The mutant ParE proteins could be divided into two groups when reconstituted with ParC to form topoisomerase IV, those that elicited hyper-DNA cleavage and those that affected covalent complex formation.
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