期刊
ANALYTICA CHIMICA ACTA
卷 407, 期 1-2, 页码 97-103出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0003-2670(99)00797-7
关键词
co-immobilization; lactate oxidase; lactate dehydrogenase; substrate recycling; amplification
Lactate oxidase (LOD) and lactate dehydrogenase (LDH) have been co-immobilized on electrochemically prepared polyaniline (PANI) films by physical adsorption. Regeneration of L-lactate by substrate recycling is shown to provide an amplification of the sensor response. The LOD/LDH immobilized electrodes show a linearity from 0.1 to 1 mM of lactate and stability of about 3 weeks at 4-10 degrees C. These PANI/LOD-LDH enzyme electrodes have a detection limit of 5 x 10(-5) M implying that these can be used for the estimation of L-lactate in cells and fermentation. A comparison of the results obtained with the PANI electrodes immobilized with either LOD or LDH, indicate that co-immobilization provides the possibility to detect L-lactate at lower concentrations. (C)2000 Elsevier Science B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据