期刊
MEMORIAS DO INSTITUTO OSWALDO CRUZ
卷 95, 期 2, 页码 221-223出版社
FUNDACO OSWALDO CRUZ
DOI: 10.1590/S0074-02762000000200014
关键词
monocytes; percoll; gradient-isolation
资金
- NIAID NIH HHS [AI-30639] Funding Source: Medline
We describe a simple method using percoll gradient for isolation of highly enriched human monocytes. High numbers of fully functional cells are obtained from whole blood or buffy, coat cells. The use of simple laboratory equipment and a relatively cheap I reagent makes the described method a convenient approach to obtaining human monocytes.
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