期刊
BIOMEDICAL CHROMATOGRAPHY
卷 29, 期 4, 页码 619-625出版社
WILEY-BLACKWELL
DOI: 10.1002/bmc.3323
关键词
phenethyl isothiocyanate (PEITC); LC-APCI-MS; MS; human plasma; pharmacokinetics
Phenethyl isothiocyanate (PEITC) is a promising chemopreventive agent present in cruciferous vegetables. This paper describes the development of a method for the determination of PEITC in human plasma by liquid chromatography/tandem mass spectrometry (LC-MS/MS). Atmospheric-pressure chemical ionization was found more suitable for ionization of PEITC than electrospray ionization. Because of the lability of PEITC, a combination of low temperature and acidification was applied to minimize the degradation during the sample collection and preparation procedure. A simple protein precipitation with acetonitrile was used for the preparation of plasma samples. The analyte and 1-phenylpropyl isothiocyanate as internal standard (IS) were subjected to chromatographic analysis on a C-18 column (50x2.1mm, 5 mu m) using 85% methanol as mobile phase at a flow rate of 0.3mL/min. The total analysis time for each chromatograph was 3min and the results were linear over the studied range (5.00-250ng/mL). The intra- and inter-day precision values were acceptable as per US Food and Drug Administration guidelines. This method was successfully applied in the determination of PEITC concentrations in plasma samples from healthy chinese Volunteers. Copyright (c) 2014 John Wiley & Sons, Ltd.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据