期刊
BIOMEDICAL CHROMATOGRAPHY
卷 27, 期 12, 页码 1767-1774出版社
WILEY
DOI: 10.1002/bmc.2991
关键词
clematichinenoside AR; intestinal microflora; metabolite identification; LC-Q/TOF MS
类别
资金
- National Center of Create New Drug Major Projects of China [2009ZX09103-371]
- Jiangsu Provincial High Technology Research and Development Program of China [BG2007613]
- Project of Traditional Chinese Medicine Administration Bureau of Jiangsu [LB09059]
Clematichinenoside AR (C-AR), a pentacyclic triterpenoid saponin with anti-inflammatory and anti-rheumatoid activities, is the main active component of the traditional Chinese medicine Clematidis Radix et Rhizoma. However, its poor oral absorption indicated that not only the parent compound C-AR itself, but also its metabolites could be responsible for the pharmacological effects in rats. The present study aimed to investigate the metabolism of C-AR in rat intestinal microflora, where C-AR was extensively metabolized. C-AR was incubated with the content of the large intestine. The culture solution was collected at different time points and analyzed for the metabolites of C-AR. Eight metabolites were identified by liquid chromatography/quadrupole time-of-flight mass spectrometry. M1, M2 and M5 were the major metabolites. In addition, it was proposed that deglycosylation was the only pathway contributing to the biotransformation of C-AR in rat intestinal microflora. Copyright (c) 2013 John Wiley & Sons, Ltd.
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