期刊
MECHANISMS OF DEVELOPMENT
卷 91, 期 1-2, 页码 249-258出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0925-4773(99)00309-3
关键词
mouse embryonic development; mesoderm formation; embryonic stem cells; Wnt/beta-catenin signaling pathway; Brachyury
To identify target genes of the Wnt/beta-catenin signaling pathway in early mouse embryonic development we have established a co-culture system consisting of NIH3T3 fibroblasts expressing different Wnts as feeder layer cells and embryonic stem (ES) cells expressing a green fluorescent protein (GFP) reporter gene transcriptionally regulated by the TCF/beta-catenin complex. ES cells specifically respond to Wnt signal as monitored by GFP expression. In GFP-positive ES cells we observe expression of Brachyury. Two TCF binding sites located in a 500 bp Brachyury promoter fragment bind the LEF-1/beta-catenin complex and respond specifically to beta-catenin-dependent transactivation. From these results we conclude that Brachyury is a target gene for Wnt/beta-catenin signaling. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
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