Quantitative determination of bioactive alkaloids lysergol and chanoclavine in Ipomoea muricata by reversed-phase high-performance liquid chromatography

A rapid, simple, sensitive, gradient and reproducible, reverse-phase high-performance liquid chromatographic method was developed for the quantitative estimation of bioactive alkaloids, lysergol and chanoclavine in the seeds of Ipomoea muricata. The clavine alkaloid, lysergol, is a bioenhancer for the drugs and nutrients. The samples were analyzed by reverse-phase chromatography on a Waters spherisorb ODS2 column (250?x?4.6?mm, i.d., 10?mu m) using binary gradient elution with acetonitrile and 0.01?m phosphate buffer (NaH2PO4) containing 0.1% glacial acetic acid at a flow rate of 0.8?mL/min, a column temperature of 25 degrees C and UV detection at ? 254?nm. The limits of detection (LOD) and quantitation (LOQ) were 0.035 and 0.106?mu g/mL for lysergol and 0.039 and 0.118?mu g/mL for chanoclavine, respectively. Standard curves were linear in the range of 210?mu g/mL (r?>?99) for both analytes. Good results were achieved with respect to repeatability (RSD?