LC-MS/MS method for the quantification of myo- and chiro-inositol as the urinary biomarkers of insulin resistance in human urine

A simple LC-MS/MS method has been developed and validated for the quantification of endogenous myo- and chiro-inositol in human urine. myo- and chiro-Inositol were completely resolved from other carbohydrates and there were no interference peaks in human urine. The correlation coefficient (n?=?3) was greater than 0.9991 over the range 0.0525.0?mu g/mL with the weighted (1/C2) least square method. Precision (%RSD) and accuracy (%RE) were 010.0% and 06.0% for the intra-day assay (n?=?5) and 014.3% and 010.0% for the inter-day assay (n?=?5). myo- and chiro-Inositol have been shown to be stable in human urine stored at room temperature and for three freezethaw cycles. Copyright (c) 2011 John Wiley & Sons, Ltd.