期刊
BIOMEDICAL CHROMATOGRAPHY
卷 23, 期 11, 页码 1201-1207出版社
WILEY
DOI: 10.1002/bmc.1244
关键词
curcumin; HPLC; 96-well protein precipitation; pharmacokinetics
类别
资金
- Science and Technology Department of Shanghai, P. R. China [04DZ19216]
This paper describes a sensitive, specific and rapid high-performance liquid chromatography (HPLC) method for the determination of curcumin in rat plasma. After a simple step of protein precipitation in 96-well format using acetonitrile containing the internal standard (IS), emodin, plasma samples were analyzed by reverse-phase HPLC. Curcumin and the IS emodin were separated on a Diamonsil C-18 analytical column (4.6 x 100 mm, 5 mu m) using acetonitrile-5% acetic acid (75:25, v/v) as mobile phase at a flow rate of 1.0 mL/min. The method was sensitive with a lower limit of quantitation of 1 ng/mL, with good linearity (r(2) >= 0.999) over the linear range 1-500 ng/mL. All the validation data, such as accuracy and precision, were within the required limits. A run time of 3.0 min for each sample made high-throughput bioanalysis possible. The assay method was successfully applied to the study of the pharmacokinetics of curcumin liposome in rats. Copyright (C) 2009 John Wiley & Sons, Ltd.
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