期刊
MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
卷 466, 期 1, 页码 1-7出版社
ELSEVIER
DOI: 10.1016/S1383-5718(99)00228-4
关键词
titanium dioxide (TiO2); UVA; DNA strand breaking; hydroxyl radical; active oxygen; polyhydric alcohols
We investigated the photodynamic DNA strand-breaking activity of TiO2. A solution of super-coiled pBR 322 DNA was irradiated with 5 J/cm(2) of UVA in the presence of TiO2 and the products were analyzed by using gel electrophoresis. The ratio of open-circular DNA to super-coiled circular DNA was calculated from the resulting peak areas as a DNA strand-breaking index (SBI). The SBI of anatase-structure TiO2 (band gap = 3.23 eV) was greater than that of rutile structure (band gap = 3.06 eV), and the level of SBI correlated with the photocatalytic activity for degradation of 2-propanol. The inhibitory effects of active oxygen scavengers, including DMSO, glutathione and histidine, on the DNA strand-breaking activity were examined. All of the scavengers except ascorbic acid showed inhibitory effects, as did several polyhydric alcohols including mannitol, a well-known hydroxyl radical scavenger. These results suggest that the photodynamic DNA strand-breaking activity of TiO2 is due to active oxygen species, especially hydroxyl radicals. Polyhydric alcohols showed an inverse correlation between the inhibitory effect on DNA strand-breaking activity and the octanol/water partition coefficient (log P). (C) 2000 Elsevier Science B.V. All rights reserved.
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