期刊
TALANTA
卷 51, 期 3, 页码 415-439出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/S0039-9140(99)00294-5
关键词
enhanced chemiluminescence; chemiluminogenic labels; chemiluminescent assays; oxygen metabolites
The principles of chemiluminescence and its applications as a diagnostic tool are reviewed. After an introduction to the theoretical aspects of luminescence and energy transfer, the different classes of chemiluminogenic labels including luminol, acridinium compounds, coelenterazine and analogues, dioxetanes, systems based on peroxyoxalic acid and their derivatives are described emphasizing the molecules which best fulfil the requirements of today's clinical chemistry. Applications of chemiluminescence and enhanced chemiluminescence to immunoassays, receptor assays, DNA probes, biosensors and oxygen metabolism are discussed as well as the role of enzymes in the selectivity and the sensitivity of these reactions. (C) 2000 Elsevier Science B.V. All rights reserved.
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