期刊
BIOMEDICAL CHROMATOGRAPHY
卷 22, 期 12, 页码 1385-1392出版社
WILEY
DOI: 10.1002/bmc.1070
关键词
Shaofu Zhuyu decoction; bioactive components; human umbilical vein endothelial cell extraction; HPLC-Q-TOF-MS
类别
资金
- 2006 Great Basic Science Research Project of Jiangsu College and University [06KJA36022]
- 2006 'Qinglan Project' Scientific and Technological Innovation Team Training Program of Jiangsu College and University
In this paper, a useful method for screening and analyzing the potential bioactive components in bioassay-guided fraction (SF-11) from Shaofu Zhuyu decoction was developed using human umbilical vein endothelial cell (HUVEC) extraction and high-performance liquid chromatography coupled with Q-TOF/MS spectrometry. In addition, the protective effects on HUVEC damage induced by adrenaline in vitro were also investigated. The results showed that SF-11 significantly inhibited the endothelin (ET) release and reversed the NO secretion of HUVEC (p < 0.05), and promoted the PGI(2) release of HUVEC (p < 0.05). Two effective components, paeoniflorin and typhaneoside, from SF-11. were screened and identified using live cell extract and HPLC coupled with Q-TOF/MS spectrometry. The compounds, paeoniflorin and typhaneoside, showed significantly inhibiting effects on the ET release and reversing of NO secretion of HUVEC (p < 0.05), with similar effects to SF-11, and promoting the PGI(2) release of HUVEC at the concentration of 0.208 and 0.013 mu mol/mL, respectively (p < 0.05). These data indicated that the method of live cell extraction coupled with HPLC-MS technology is feasible, rapid and useful for screening and analyzing potential bioactive components from TCMs. Copyright (C) 2008 John Wiley & Sons, Ltd.
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