期刊
BIOMEDICAL CHROMATOGRAPHY
卷 22, 期 11, 页码 1279-1287出版社
JOHN WILEY & SONS LTD
DOI: 10.1002/bmc.1058
关键词
pretreatment method; ultrafiltration membrane; mouse plasma; amyloid beta-protein fragment; new gradient system; acetic acid
During the evaluation of a pretreatment method for the simultaneous quantification of four amyloid beta-protein fragments in transgenic mice plasma by a new gradient system, we have found that acetic acid has potency to completely dissolve plasma polypeptides in the presence of an organic solvent. Based on this observation, we designed a simple pretreatment method using an ultrafiltration membrane. An analysis of the filtrate obtained by this method suggests the possibility that acetic acid inhibits the interaction between amyloid beta-protein fragments and plasma polypeptides, which leads to a higher recovery of the amyloid,beta-protein fragments from mouse plasma. In addition, higher dilution of mouse plasma using a dilution solution produced higher recovery as well. The highest recovery of amyloid beta-protein 1-38, 1-40, 1-42 and 1-43 fragments was 101.7, 94.9, 96.2 and 84.8%, respectively. Furthermore, calibration curves with the lower limit of quantification of 0.65 nM were successfully constructed with good accuracy using the developed method. Consequently, a pretreatment method using an ultrafiltration membrane is a powerful tool to determine the amyloid P-protein fragments in transgenic mice plasma containing an abundance of plasma polypeptides such as albumin. Copyright (c) 2008 John Wiley & Sons, Ltd.
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