期刊
BIOMATERIALS
卷 35, 期 8, 页码 2477-2487出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.11.044
关键词
Injectable; Gelatin; Cryogel; Degradable; Matrix metalloproteinase
资金
- NIH [R01 EB015498]
- NSERC PGS-D
- HHMI ISRF
- National Science Foundation under NSF [ECS-0335765]
The performance of biomaterials-based therapies can be hindered by complications associated with surgical implant, motivating the development of materials systems that allow minimally invasive introduction into the host. In this study, we created cell-adhesive and degradable gelatin scaffolds that could be injected through a conventional needle while maintaining a predefined geometry and architecture. These scaffolds supported attachment, proliferation, and survival of cells in vitro and could be degraded by recombinant matrix metalloproteinase-2 and -9. Prefabricated gelatin cryogels rapidly resumed their original shape when injected subcutaneously into mice and elicited only a minor host response following injection. Controlled release of granulocyte-macrophage colony-stimulating factor from gelatin cryogels resulted in complete infiltration of the scaffold by immune cells and promoted matrix metalloproteinase production leading to cell-mediated degradation of the cryogel matrix. These findings suggest that gelatin cryogels could serve as a cell-responsive platform for biomaterial-based therapy. (C) 2013 Elsevier Ltd. All rights reserved.
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