期刊
BIOMATERIALS
卷 35, 期 8, 页码 2401-2410出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.11.037
关键词
Human mesenchymal stem cells; Differentiation; Nanotopography; Osteogenesis; Adipogenesis; Capillary force lithography
资金
- National Institutes of Health [R21EB008562-01A1, U54CA141868, P50CA10 3175]
- Basic Science Research Program through the National Research Foundation of Korea [2010-0010840]
- Ministry of Education, Science and Technology
- National Institute of Environmental Health Sciences (NIEHS)
- University of Washington Center for Ecogenetics and Environmental Health
- ITHS [NIH/NIEHS P30ES007033]
- National Heart, Lung, and Blood Institute [HL107361]
- Department of Bioengineering at the University of Washington
- National Research Foundation of Korea [2010-0010840] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Adult stem cells hold great promise as a source of diverse terminally differentiated cell types for tissue engineering applications. However, due to the complexity of chemical and mechanical cues specifying differentiation outcomes, development of arbitrarily complex geometric and structural arrangements of cells, adopting multiple fates from the same initial stem cell population, has been difficult. Here, we show that the topography of the cell adhesion substratum can be an instructive cue to adult stem cells and topographical variations can strongly bias the differentiation outcome of the cells towards adipocyte or osteocyte fates. Switches in cell fate decision from adipogenic to osteogenic lineages were accompanied by changes in cytoskeletal stiffness, spanning a considerable range in the cell softness/rigidity spectrum. Our findings suggest that human mesenchymal stem cells (hMSC) can respond to the varying density of nanotopographical cues by regulating their internal cytoskeletal network and use these mechanical changes to guide them toward making cell fate decisions. We used this finding to design a complex two-dimensional pattern of co-localized cells preferentially adopting two alternative fates, thus paving the road for designing and building more complex tissue constructs with diverse biomedical applications. (C) 2013 Elsevier Ltd. All rights reserved.
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