期刊
BIOCHEMISTRY
卷 39, 期 11, 页码 3106-3114出版社
AMER CHEMICAL SOC
DOI: 10.1021/bi992710r
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资金
- NIGMS NIH HHS [GM57500] Funding Source: Medline
Twelve classes of deoxyribozymes that promote an ATP-dependent self-capping reaction were isolated by in vitro selection from a random-sequence pool of DNA. Each deoxyribozyme catalyzes the transfer of the AMP moiety of ATP to its 5'-terminal phosphate group, thereby forming a 5',5'-pyrophosphate linkage. An identical DNA adenylate structure is generated by the T4 DNA Ligase during enzymatic DNA ligation. A 41-nucleotide class I deoxyribozyme requires Cu2+ as a cofactor and adopts a structure that recognizes both the adenine and triphosphate moieties of ATP or dATP, The catalytic efficiency for this DNA, measured at 10(4) M(-1.)min(-1) using either ATP or dATP as substrate, is similar to other catalytic nucleic acids that use small substrates. Chemical probing and site-directed mutagenesis implicate the formation of guanine quartets as critical components of the active structure, The observation of ATP-dependent 'self-charging by DNA suggests that DNA could be made to perform the reactions typically associated with DNA cloning, but without the assistance of protein enzymes.
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