期刊
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
卷 269, 期 3, 页码 704-708出版社
ACADEMIC PRESS INC
DOI: 10.1006/bbrc.2000.2335
关键词
neurotensin; G-protein receptor; MAPK; AP-1; signal transduction; gut hormone
资金
- NCI NIH HHS [F32CA79187] Funding Source: Medline
- NIA NIH HHS [R01AG10885] Funding Source: Medline
- NIDDK NIH HHS [P01DK35608] Funding Source: Medline
Neurotensin (NT), a gastrointestinal (GI) hormone, binds its receptor (NTR) to stimulate proliferation of normal and neoplastic GI tissues; the molecular mechanisms remain largely undefined. Mitogen-activated protein kinases (MAPKs) are a family of intracellular kinases that transmit mitogenic signals by translocating to the nucleus and activating transcription factors. The purposes of this study were: (1) to identify whether the MAPKs (ERK1/2 and JNK) are activated by NT and (2) to determine the effect of NT on downstream transcription factors using the human pancreatic adenocarcinoma cell line, MIA PaCa-2, which possesses high-affinity NTR. Both ERK and JNK activity were stimulated within 3-6 min by treatment with NT (10 nM); steady-state levels of ERK and JNK protein were unchanged. Moreover, NT treatment resulted in increased AP-1 binding activity as determined by gel shift analysis. Delineating the signal transduction mechanisms regulating the cellular effects of NT will provide important insights into the molecular pathways responsible for NT-mediated effects on both normal and neoplastic cells, (C) 2000 Academic Press.
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