期刊
BIOMATERIALS
卷 35, 期 20, 页码 5407-5413出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.03.040
关键词
Dendrimer; Poly(propylenimine); Gene transfection; Fluorination
资金
- National Natural Science Foundation of China, China [21322405]
- Shanghai Rising-star Program [13QA1401500]
- Science and Technology of Shanghai Municipality [11DZ2260300]
The use of poly(propylenimine) (PPI) dendrimers as gene delivery vectors is limited by their low transfection efficacy and serious cytotoxicity. In this study, we prepare a series of efficient and low cytotoxic gene vectors based on PPI dendrimers using a facile fluorination strategy. Fluorinated G3, G4, and G5 PPI dendrimers show comparable or superior transfection efficacies to six representative transfection reagents such as Lipofectamine 2000, jetPEI, branched poly(ethyleneimine), SuperFect, PolyFect, as well as arginine-modified dendrimer on both HEK293 and HeLa cells. These fluorinated PPI dendrimers exhibit low cytotoxicity on the transfected cells because they achieve optimal transfection efficacy at extremely low nitrogen to phosphorus (N/P) ratios. In addition, the synthesized materials show high transfection efficacy on 3D multicellular spheroids. These results demonstrate that fluorination is a promising strategy to improve the transfection efficacy of PPI dendrimers while reducing the cytotoxicity of these cationic polymers. (C) 2014 Elsevier Ltd. All rights reserved.
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