期刊
BIOMATERIALS
卷 35, 期 24, 页码 6299-6310出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.04.044
关键词
Magnesium; Osteoclast; Osteolysis; NF-kappa B; NFATc1
资金
- Key National Basic Research Program of China [2012CB619101]
- Major Basic Research of Science and Technology Commission of Shanghai Municipality [11DJ1400303]
- Science and Technology Commission of Shanghai Municipality [11DJ1400303]
- National Natural Science Foundation of China [81190133]
- National Natural Science Foundation for the Youth of China [81201364]
- scientific research grant for Youth of Shanghai [ZZjdyx 2097]
- scientific research grant from 985 project stem cell and regenerative medicine centre
- Shanghai Municipal Education Commission [13YZ031]
- Chinese Academy of Sciences [XDA01030502]
- Doctoral Innovation Foundation from Shanghai Jiaotong University School of Medicine [BXJ201330]
Wear particle-induced aseptic prosthetic loosening is one of the most common reasons for total joint arthroplasty (TJA). Extensive bone destruction (osteolysis) by osteoclasts plays an important role in wear particle-induced pen-implant loosening. Thus, strategies for inhibiting osteoclast function may have therapeutic benefit for prosthetic loosening. Here, we mimicked the process of magnesium (Mg) degradation in vivo and obtained Mg leach liquor (MLL) by immersing pure Mg in culture medium. For the first time, we demonstrated that MLL suppresses osteoclast formation, polarization, and osteoclast bone resorption in vitro. An in vivo assay demonstrated that MLL attenuates wear particle-induced osteolysis. Furthermore, we found that MLL significantly inhibits nuclear factor-kappa B (NF-kappa B) activation by retarding inhibitor-kappa B degradation and subsequent NF-kappa B nuclear translocation. We also found that MLL attenuates the expression of NFATc1 at both the protein and mRNA levels. These results demonstrate that MLL has anti-osteoclast activity in vitro and prevents wear particle-induced osteolysis in vivo. Collectively, our study suggests that metallic magnesium, one of the orthopedic implants with superior properties, has significant potential for the treatment of osteolysis-related diseases caused by excessive osteoclast formation and function. (C) 2014 Elsevier Ltd. All rights reserved.
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