期刊
BIOMATERIALS
卷 35, 期 15, 页码 4525-4535出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.02.008
关键词
Polyelectrolyte multilayer film; Drug delivery; SDF-1 alpha/CXCL12; CXCR4; Myoblast; Migration
资金
- ARC (Association Recherche contre le Cancer) foundation [PDF20121206052]
- Canceropole Lyon Auvergne Rhone-Alpes (CLARA)
- Canceropole Lyon Auvergne Rhone-Alpes (MICAN)
- ANR [NT05-4_41976]
- European Commission via a European Research Council [GA 259370]
Several chemokines are important in muscle myogenesis and in the recruitment of muscle precursors during muscle regeneration. Among these, the SDF-1 alpha chemokine (CXCL12) is a potent chemoattractant known to be involved in muscle repair. SDF-1 alpha was loaded in polyelectrolyte multilayer films made of poly(L-lysine) and hyaluronan to be delivered locally to myoblast cells in a matrix-bound manner. The adsorbed amounts of SDF-1 alpha were tuned over a large range from 100 ng/cm(2) to 5 mu g/cm(2), depending on the initial concentration of SDF-1 alpha in solution, its pH, and on the film crosslinking extent. Matrix-bound SDF-1 alpha induced a striking increase in myoblast spreading, which was revealed when it was delivered from weakly crosslinked films. It also significantly enhanced cell migration in a dose-dependent manner, which again depended on its presentation by the biopolymeric film. The low-crosslinked film was the most efficient in boosting cell migration. Furthermore, matrix-bound SDF-1 alpha also increased the expression of myogenic markers but the fusion index decreased in a dose-dependent manner with the adsorbed amount of SDF-1 alpha. At high adsorbed amounts of SDF-1 alpha, a large number of Troponin T-positive cells had only one nucleus. Overall, this work reveals the importance of the presentation mode of SDF-1 alpha to emphasize its effect on myogenic processes. These films may be further used to provide insight into the role of SDF-1 alpha presented by a biomaterial in physiological or pathological processes. (C) 2014 Elsevier Ltd. All rights reserved.
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