期刊
BIOMATERIALS
卷 35, 期 26, 页码 7286-7294出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.05.043
关键词
Functional surfaces; Planar solid-supported polymer membranes; Membrane protein; Biomolecule insertion; Amphiphilic block copolymers
资金
- Swiss National Science Foundation
- University of Basel
Planar solid-supported membranes based on amphiphilic block copolymers represent promising systems for the artificial creation of structural surfaces. Here we introduce a method for engineering functional planar solid-supported membranes through insertion of active biomolecules. We show that membranes based on poly(dimethylsiloxane)-block-poly(2-methyl-2-oxazoline) (PDMS-b-PMOXA) amphiphilic diblock copolymers, which mimic natural membranes, are suitable for hosting biomolecules. Our strategy allows preparation of large-area, well-ordered polymer bilayers via Langmuir-Blodgett and Langmuir-Schaefer transfers, and insertion of biomolecules by using Bio-Beads. We demonstrate that a model membrane protein, the potassium channel from the bacterium Mesorhizobium loti, remains functional after insertion into the planar solid-supported polymer membrane. This approach can be easily extended to generate a platform of functional solid-supported membranes by insertion of different hydrophobic biomolecules, and employing different types of solid substrates for desired applications. (C) 2014 Elsevier Ltd. All rights reserved.
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