期刊
BIOMATERIALS
卷 35, 期 5, 页码 1507-1518出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.11.014
关键词
Macrophage; Immunoreaction; Bone substitute; beta-TCP; Osteogenesis
资金
- Australian Research Council [DP120103697]
- Recruitment Program of Global Young Talent, China
- Natural Science Foundation of China [81201202, 31370963]
Immune reactions play important roles in determining the in vivo fate of bone substitute materials, either in new bone formation or inflammatory fibrous tissue encapsulation. The paradigm for the development of bone substitute materials has been shifted from inert to immunomodulatory materials, emphasizing the importance of immune cells in the material evaluation. Macrophages, the major effector cells in the immune reaction to implants, are indispensable for osteogenesis and their heterogeneity and plasticity render macrophages a primer target for immune system modulation. However, there are very few reports about the effects of macrophages on biomaterial-regulated osteogenesis. In this study, we used beta-tricalcium phosphate (beta-TCP) as a model biomaterial to investigate the role of macrophages on the material stimulated osteogenesis. The macrophage phenotype switched to M2 extreme in response to beta-TCP extracts, which was related to the activation of calcium-sensing receptor (CaSR) pathway. Bone morphogenetic protein 2 (BMP2) was also significantly upregulated by the beta-TCP stimulation, indicating that macrophage may participate in the beta-TCP stimulated osteogenesis. Interestingly, when macrophage-conditioned beta-TCP extracts were applied to bone marrow mesenchymal stem cells (BMSCs), the osteogenic differentiation of BMSCs was significantly enhanced, indicating the important role of macrophages in biomaterial-induced osteogenesis. These findings provided valuable insights into the mechanism of material-stimulated osteogenesis, and a strategy to optimize the evaluation system for the in vitro osteogenesis capacity of bone substitute materials. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据