期刊
BIOMATERIALS
卷 35, 期 26, 页码 7295-7307出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.05.033
关键词
Mesenchymal stem cells (MSCs); Coculture; Endothelial progenitor cells (EPCs); Cell-cell interaction; Integrin; Notch signaling
资金
- Program for Stem Cell and Regenerative Medicine Frontier Research - National Science Council Taiwan (NSC) [NSC102-2321-B-002-033]
The structural evolution of three-dimensional spheroids self-assembled from two different types of cells on selective biomaterials is demonstrated in this study. The two types of cells involved in the self-assembly are human mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs). When seeded in different population ratios, they can create a variety of cellular patterns on different biomaterial substrates. When the two populations are matched in initial numbers, they are self-assembled in co-spheroids with different morphologies (i.e. randomly mixed, bumped, or concentric spheroids). The morphologies are influenced by the specific cell-substrate interaction possibly through integrin signaling, as well as a substrate-dependent regulation of heterophilic cell-cell interaction possibly through Notch signaling. In particular, the self-assembled core-shell concentric spheroids from adipose-derived MSCs and EPCs show a greater angiogenic effect in vitro. This study reveals the possibility to modulate the self-assembled morphology as well as the effect of cocultured cells by changing the cell culture substratum. (C) 2014 Elsevier Ltd. All rights reserved.
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