期刊
BIOMATERIALS
卷 35, 期 29, 页码 8321-8329出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.05.096
关键词
Graphene; Reprogramming; Induced pluripotent stem cells; Mesenchymal-to-epithelial transition
资金
- National Research Foundation of Korea - Ministry of Education, Science, and Technology [NRF-2013R1A1A1058835, NRF-2013M3A9B4076485, NRF-2013M3A9B4044387]
- Korea Health Technology R&D Project, Ministry of Health Welfare [HI13C0540]
- Next-Generation BioGreen 21 Program, Rural Development Administration [PJ009073]
Graphene has been attracting considerable interest in the field of biomedical engineering because graphene and its derivatives are considered to be ideal platforms for supporting cell growth and differentiation. Here we report that graphene promotes the reprogramming of mouse somatic fibroblasts into induced pluripotent stem cells (iPSCs). We constructed a layer of graphene film on a glass substrate and characterized it as a monolayer using Raman spectroscopy. We found that the graphene substrate significantly improved cellular reprogramming efficiency by inducing mesenchymal-to-epithelial-transition (MET) which is known to affect H3K4me3 levels. Thus, our results reveal that a graphene substrate directly regulates dynamic epigenetic changes associated with reprogramming, providing an efficient tool for epigenetic pluripotent reprogramming. (C) 2014 Elsevier Ltd. All rights reserved.
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