4.8 Article

Matrix elasticity, replicative senescence and DNA methylation patterns of mesenchymal stem cells

期刊

BIOMATERIALS
卷 35, 期 24, 页码 6351-6358

出版社

ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.04.079

关键词

Mesenchymal stem cells; Elasticity; Long-term culture; Platelet lysate; DNA-methylation; Epigenetic

资金

  1. German Research Foundation (DFG) within Boost-Fund Project MechCell of the excellence initiative of RWTH Aachen University [WA 1706/3-2]
  2. Else Kroner-Fresenius Stiftung
  3. Stem Cell Network North Rhine Westphalia

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Matrix elasticity guides differentiation of mesenchymal stem cells (MSCs) but it is unclear if these effects are only transient - while the cells reside on the substrate - or if they reflect persistent lineage commitment. In this study, MSCs were continuously culture-expanded in parallel either on tissue culture plastic (TCP) or on polydimethylsiloxane (PDMS) gels of different elasticity to compare impact on replicative senescence, in vitro differentiation, gene expression, and DNA methylation (DNAm) profiles. The maximal number of cumulative population doublings was not affected by matrix elasticity. Differentiation towards adipogenic and osteogenic lineage was increased on soft and rigid biomaterials, respectively but this propensity was no more evident if cells were transferred to TCP. Global gene expression profiles and DNAm profiles revealed relatively few differences in MSCs cultured on soft or rigid matrices. Furthermore, only moderate DNAm changes were observed upon culture on very soft hydrogels of human platelet lysate. Our results support the notion that matrix elasticity influences cellular behavior while the cells reside on the substrate, but it does not have major impact on cell-intrinsic lineage determination, replicative senescence or DNAm patterns. (C) 2014 Elsevier Ltd. All rights reserved.

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