期刊
BIOMATERIALS
卷 35, 期 33, 页码 9137-9143出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2014.07.037
关键词
Brain tumor initiating cells; Epithelial-to-mesenchymal transition; Hyaluronic acid; Microenvironment
资金
- National Institutes of Health [R01CA172455]
- NIH training grant [T32CA138312]
- American Brain Tumor Association Fellowship
- Egtvedt Fellowship
Emerging evidence implicates cancer stem cells (CSCs) as primary determinants of the clinical behavior of human cancers, representing an ideal target for next-generation anti-cancer therapies. However CSCs are difficult to propagate in vitro, severely limiting the study of CSC biology and drug development. Here we report that growing cells from glioblastoma (GEM) cell lines on three dimensional (3D) porous chitosan-alginate (CA) scaffolds dramatically promotes the proliferation and enrichment of cells possessing the hallmarks of CSCs. CA scaffold-grown cells were found more tumorigenic in nude mouse xenografts than cells grown from monolayers. Growing in CA scaffolds rapidly promoted expression of genes involved in the epithelial-to-mesenchymal transition that has been implicated in the genesis of CSCs. Our results indicate that CA scaffolds have utility as a simple and inexpensive means to cultivate CSCs in vitro in support of studies to understand CSC biology and develop more effective anti-cancer therapies. (C) 2014 Elsevier Ltd. All rights reserved.
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