期刊
BIOMATERIALS
卷 34, 期 2, 页码 470-480出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.09.054
关键词
Dendrimers; Gold nanoparticles; Computed tomography; Folic acid; Lung adencarcinoma
资金
- National Natural Science Foundation of China [30901730, 81270032, 81271384, 20974019]
- Innovation Funds of Donghua University Doctorate Dissertation of Excellence [BC201102]
- High-Tech Research and Development Program of China [2012AA030309]
- Ph.D. Programs Foundation of Ministry of Education of China [20090073110072]
- Doctoral Innovation Fund of Shanghai Jiaotong University School of Medicine [BXJ201043]
- Nano Specialized Research Fund of Shanghai Science and Technology Commission [1052nm05800]
- Shanghai Jiao Tong University medical engineering crossover Fund Project [YG2011MS47]
- Natural Science Foundation of Shanghai Science and Technology Commission [12ZR1424900]
- Key Program of Science and Technology Commission of Shanghai Municipality [11JC1410500]
- National Science & Technology Pillar Program [2011BAI08B10]
We report a new usage of folic acid-modified dendrimer-entrapped gold nanoparticles (Au DENPs-FA) as nanoprobes for in vitro and in vivo targeted computed tomography (CT) imaging of human lung adencarcinoma. In this study, Au DENPs prepared using amine-terminated generation 5 poly(amidoamine) dendrimers as templates were covalently linked with FA, followed by an acetylation reaction to neutralize the remaining dendrimer surface amines. The formed Au DENPs-FA was used for both in vitro and in vivo targeted CT imaging of human lung adencarcinoma cells (SPC-A1 cells) and the xenograft tumor model, which express folic acid receptors (FAR) verified by immunohistochemical staining. Micro-CT images show that SPC-A1 cells can be detected under X-ray after incubation with the Au DENPs-FA in vitro and the xenograft tumor model can be imaged after intravenous, intratumoral, and intraperitoneal administration of the particles. Transmission electron microscopy data confirm that the Au DENPs-FA is able to be uptaken dominantly in the lysosomes of the cells. Combined morphological observation of cells after Hematoxylin and Eosin staining, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay of cell viability, and flow cytometric analysis of cell cycle and apoptosis show that the Au DENPs-FA does not affect cell morphology, viability, and cell cycle and apoptosis, indicating their good biocompatibility at the given concentration range. These findings suggest that the developed Au DENPs-FA have a great potential to be used as imaging probes for targeted CT imaging of human lung adencarcinoma. (C) 2012 Elsevier Ltd. All rights reserved.
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