Comparative in vitro isolation of Trypanosoma theileri from cattle in Belgium

Ten blood samples randomly collected from cows on a farm nearby Antwerp, Belgium, were inoculated into KIVI culture medium (Kit for In Vitro Isolation of trypanosomes) and RPMI 10% + feeder medium. Within 3 weeks of incubation all KIVI cultures and four RPMI 10% + feeder revealed presence of Trypanosoma theileri. Same practical implications regarding the use of KIVI for isolation of pathogenic African trypanosomes from cattle and other Bovidae are discussed. (C) 2000 Elsevier Science B.V. All rights reserved.