期刊
BIOMATERIALS
卷 34, 期 22, 页码 5711-5721出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.04.007
关键词
pH-Sensitive polymer; pH-sensitive liposome; Cytoplasmic delivery; Cancer immunotherapy; Dendritic cell; Cellular immunity
资金
- Ministry of Education, Science, Sports, and Culture in Japan
- Japan Science and Technology Agency
- Grants-in-Aid for Scientific Research [25750177, 23240075] Funding Source: KAKEN
For production of pH-sensitive liposomes, we developed pH-sensitive polymer-lipids that consists of pH-sensitive fusogenic polymer moieties such as 3-methyl glutarylated poly(glycidol) and 2-carboxycyclohexane-1-carboxylated poly(glycidol), connected to a phosphatidylethanolamine head group. Incorporation of these pH-sensitive polymer-lipids into egg yolk phosphatidylcholine liposomes produced highly pH-sensitive liposomes that were stable at neutral pH but which destabilized markedly in response to very small pH change in weakly acidic pH region. These liposomes delivered their contents (pyranine) into cytosol of dendritic cell-derived DC2.4 cells. When these polymer-lipid-incorporated liposomes loaded with antigenic protein ovalbumin (OVA) were administered subcutaneously to mice, the antigen-specific cellular immunity was induced efficiently in the mice. Furthermore, immunization of mice with these OVA-loaded pH-sensitive polymer-lipid-incorporated liposomes induced strong OVA-specific immunity, which achieved complete rejection of OVA-expressing E.G7-OVA cells and marked regression of E.G7-OVA tumors. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据