期刊
BIOMATERIALS
卷 34, 期 13, 页码 3256-3269出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.01.028
关键词
Acellular matrix; Age; Epithelial cell; Extracellular matrix (ECM); Lung; Mesenchymal stem cell
资金
- NIH ARRA [RC4HL106625]
- NHLBI [R21HL094611]
- UVM Lung Biology Training grant from the NHLBI [T32 HL076122]
- UVM Environmental Pathology Training grant from the NIEHS [T32 ES007122]
- Vermont Genetics Network through from the INBRE Program of the National Institute of General Medical Sciences (NIGMS) [8P20GM103449]
Use of de-cellularized cadaveric lungs as 3-dimensional scaffolds for ex vivo lung tissue generation offers a new potential therapeutic approach for clinical lung transplantation. However, it is likely that some of the available cadaveric human lungs may be from older donors or from donors with previously existing structural lung diseases such as emphysema or pulmonary fibrosis. It is not known whether these lungs will be suitable for either de-cellularization or re-cellularization. To investigate this, we assessed the effects of advanced age, representative emphysematous and fibrotic injuries, and the combination of advanced age and emphysematous injury and found significant differences both in histologic appearance and in the retention of extracellular matrix (ECM) and other proteins, as assessed by immunohistochemistry and mass spectrometry, between the different conditions. However, despite these differences, binding, retention and growth of bone marrow-derived mesenchymal stromal cells (MSCs) over a 1-month period following intratracheal inoculation were similar between the different experimental conditions. In contrast, significant differences occurred in the growth of C10 mouse lung epithelial cells between the different conditions. Therefore, age, lung injury, and the cell type used for re-cellularization may significantly impact the usefulness of de-cellularized whole lungs for ex vivo lung tissue regeneration. (C) 2013 Elsevier Ltd. All rights reserved.
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