期刊
BIOMATERIALS
卷 34, 期 30, 页码 7335-7343出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2013.06.025
关键词
Collagen; Schwann cell; Nerve guide; Nerve regeneration; Nerve tissue engineering; Hydrogel
资金
- Medical Research Council [G113/31] Funding Source: Medline
- Medical Research Council [G113/31] Funding Source: researchfish
- MRC [G113/31] Funding Source: UKRI
A new combination of tissue engineering techniques provides a simple and effective method for building aligned cellular biomaterials. Self-alignment of Schwann cells within a tethered type-1 collagen matrix, followed by removal of interstitial fluid produces a stable tissue-like biomaterial that recreates the aligned cellular and extracellular matrix architecture associated with nerve grafts. Sheets of this engineered neural tissue supported and directed neuronal growth in a co-culture model, and initial in vivo tests showed that a device containing rods of rolled-up sheets could support neuronal growth during rat sciatic nerve repair (5 mm gap). Further testing of this device for repair of a critical-sized 15 mm gap showed that, at 8 weeks, engineered neural tissue had supported robust neuronal regeneration across the gap. This is, therefore, a useful new approach for generating anisotropic engineered tissues, and it can be used with Schwann cells to fabricate artificial neural tissue for peripheral nerve repair. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据