期刊
BIOMATERIALS
卷 34, 期 7, 页码 1757-1763出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.biomaterials.2012.11.034
关键词
Superhydrophobic surface; Micropatterning; Cell patterning; Cell signalling; Signalling molecule gradient; Polymeric materials
资金
- Young Investigator Network (YIN)
- Helmholtz Association's Initiative and Networking Fund [VH-NG-621]
- Department of Applied Physical Chemistry
- DFG Emmy Noether Grant [SCH0847-2]
- BioInterfaces Programme of the KIT
The ability to control spatial arrangement and geometry of different cell types while keeping them separated and in close proximity for a long time is crucial to mimic and study variety of biological processes in vitro. Although the existing cell patterning technologies allow co-culturing of different cell types, they are usually limited to relatively simple geometry. The methods used for obtaining complex geometries are usually applicable for patterning only one or two cell types. Here we introduce a convenient method for creating patterns of multiple (up to twenty) different cell types on one substrate. The method virtually allows any complexity of cell pattern geometry. Cell positioning on the substrate is realized by a parallel formation of multiple cell-containing microreservoirs confined to the geometry of highly hydrophilic regions surrounded by superhydrophobic borders built-in a fine nano-porous polymer film. As a case study we showed the cross-talk between two cell populations via Wnt signaling molecules propagation during co-culture in a mutual culture medium. (C) 2012 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据